Characterization of thiol-disulfide systems as candidates to combat oxidative stress
Juliana Ferreira de Santana
Gyógyszertudományok
Dr. Zelkó Romána
SE Gyógyszerészeti Intézet B ép. Zalai terem
2023-10-09 09:30:00
A gyógyszerészeti tudományok korszerű kutatási irányai
Dr. Antal István
Dr. Noszál Béla és Dr. Mirzahosseini Arash
Dr. Orosz Ádám
Dr. Sebák Fanni
Dr. Rosivall László
Dr. Fejős Ida
Dr. Fodor Blanka
Oxidative stress, known as the imbalance between prooxidants and antioxidants in
biological systems in favor of oxidants has been associated with aging, atherosclerosis,
carcinogenesis, diabetes, and neurodegeneration. The main moiety targeted by oxidant species
in the redox signaling pathways is the thiol (SH) group in the Cys residues, especially in its
deprotonated (S−
) form. 1H and 13C NMR measurements were carried outto explore anticipated
correlations between chemical shifts versus thiolate basicities and redox potentials of Cys,
CysASH, Hcy, PenA, and their homo-disulfides at the submolecular level. Cysteine-containing
small peptides were also studied. Regression analysis demonstrated a strong linear relationship
for chemical shift vs thiolate logK of the microspecies data. The closest correlation was observed
for the αCH nuclei in 1H and13C NMR data. Whereas neither site-specific basicities nor sitespecific redox potentials can be directly measured by any means in peptides and proteins
containing several thiolsand/or disulfide units, these data allow a simple method and predictive
power to estimate the above mentioned site-specific physicochemical parameters for analogous
sulfur-containing moieties in related biopolymers
