SE Anatómiai, Szövet- és Fejlődéstani Intézet könyvtára
2024-05-15 14:00:00
Embryology, Theoretical, Experimental and Clinical Developmental Biology
Dr. Nagy Nándor
Dr. Dávid Csaba
Dr. Heinzlmann Andrea
Dr. Kálmán Mihály
Dr.Arányi Zsuzsanna
Dr. Zachar Gergely
Dr. Márton Gergely
In the course of my study I made observations at three types of animals, genetically modified mice, Meriones shawi and zucker diabetic fatty rat (ZDF), optimizing possible modifications of the different sampling and stereological approaches.
I compared whole mount and radially sectioned retinas of Meriones shawi and ZDF-rats among healthy versus diabetic individuals. Compared to the regular ones, our novel modified isodensity maps eliminated the rupture artefacts effectively. The average and maximal retinal sizes are both indicated on the modified maps, thus the standard deviation of such dimensions can also be displayed.
The second part of my thesis features virtual reslicing of the microscopic slices as an other type of subsequent data modification technique, based on our previous experience. We performed this data processing at microscopic images of the posteromedial barrel field, and presented results from two different types of sectioning i.e. coronal and tangential at the same time, unprecedente in the literature. After virtual reslicing we were able to pool the data of the different sectioning. Merging the corrected image planes highlighted the spatial preference of the PV+, SST+ and VIP+ interneuron subtypes in the Layer IV barrel field only, is another novelty.
In the third part, we presented a set of stereological approaches, which lead to biological results in the examination of the somatosensory system. We found that PV+, SST+ and VIP+ cells differ at their distribution patterns. The PV+ cells do not prefer areas regarding the thalamic afferentation from VPM or POm. These cells distribute uniformly among septal vs. barrel associated areas, which contradicts the first visual impression. Novelty of our work is, that we described the non-homogenous spatial distribution of SST+ and VIP+ cells in Layer IV. These cells prefer the septum in the LIV, so they avoid the areas, where the fibers arrive from VPM. The different role of these cells can derive from the dissimilar laminar distribution. From pia mater to white matter we observed reverse distribution patterns of VIP+ and SST+ cells.
The observations on retinas and the somatosensory system were obtained by applying the image processing methods and transforming them along the stereological principles presented, and in both cases we have generated a site-specific representation.
